KMID : 1034420150400020143
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Restorative Dentistry & Endodontics 2015 Volume.40 No. 2 p.143 ~ p.148
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Effect of three different irrigation solutions applied by passive ultrasonic irrigation
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Llena Carmen
Forner Leopoldo Cambralla Raquel Lozano Adrian
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Abstract
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Objectives: This study evaluated the maximum depth and percentage of irrigant penetration into dentinal tubules by passive ultrasonic irrigation (PUI).
Materials and Methods: Thirty extracted human teeth were instrumented and divided into three groups. According to final irrigation regimen, 5.25% sodium hypochlorite (Group A, NaOCl), 2% chlorhexidine (Group B, CHX) and saline solution (Group C, control group) were applied with Irrisafe 20 tips (Acteon) and PUI. Irrigant was mixed with 0.1% rhodamine B. Sections at 2 mm, 5 mm, and 8 mm from the apex were examined with confocal laser scanning microscopy (CLSM). The percentage and maximum depth of irrigant penetration were measured. Kruskal-Wallis test and Mann-Whitney test were performed for overall comparison between groups at each level and for pairwise comparison, respectively. Within a group, Wilcoxon test was performed among different levels. p values less than 0.05 were considered significant.
Results: In all groups, highest penetration depth and percentage of penetration were observed at the 8 mm level. At 2 mm level, Groups A and B had significantly greater depths and percentages in penetration than Group C (p < 0.05), but there were no significant differences between Groups A and B. At 5 mm level, penetration depths and percentage of penetration was not significantly different among the groups.
Conclusions: NaOCl and CHX applied by PUI showed similar depth and percentage of penetration at all evaluated levels.
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KEYWORD
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Chlorhexidine, Confocal laser scanning microscope, Dentinal penetration, Passive ultrasonic irrigation, Sodium hypochlorite
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